| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 25 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | DDIT4/REDD1 Rabbit Polyclonal Antibody detects endogenous levels of DDIT4/REDD1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
REDD1 also known as DDIT4 plays a crucial role in cellular response to stress, particularly in regulating reactive oxygen species (ROS). By modulating ROS levels, REDD1 is essential for maintaining cellular homeostasis and preventing oxidative damage, which can lead to apoptosis and contribute to pathogenesis of ischemic diseases. Notably, REDD1 is an HIF-1-responsive gene significantly upregulated in hypoxic conditions, particularly in neuronal cells, highlighting REDD1′s importance in adaptive responses to low oxygen levels. This makes REDD1 a valuable target for research into neuroprotection and ischemic injury, as well as a potential biomarker for various diseases associated with oxidative stress. |
| Product Image Gallery | |
 Western blot analysis of extracts on K562, MCF7 cells, using DDIT4/REDD1 Rabbit Polyclonal Antibody (EAB13529) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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