www.ebiocell.com
| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 49 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-PTP1B (Ser50) Rabbit Polyclonal Antibody detects endogenous levels of PTP1B protein only when phosphorylated at Ser50. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PTP1B also known as PTPN1 is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTP1B plays a crucial role in regulating insulin signaling and is involved in the negative regulation of the insulin receptor, making PTP1B a significant target for understanding metabolic disorders such as obesity and diabetes. Located primarily in the endoplasmic reticulum and cytoplasm, PTP1B′s positioning is essential for function, as PTP1B interacts with various signaling pathways that modulate cellular responses to insulin. PTP1B is synthesized as a 435 amino acid precursor, which is subsequently cleaved to produce the active 321 amino acid form, highlighting the importance of post-translational modifications in activation and function. |
| Product Image Gallery | |
 Western blot analysis of extracts on COS7 cells treated with UV, using Phospho-PTP1B (Ser50) Rabbit Polyclonal Antibody (EAB13563) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
|
