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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 213 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | 53BP1 Rabbit Polyclonal Antibody detects endogenous levels of 53BP1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
53BP1 also known as TP53BP1 is a protein that functions in the DNA double-strand break repair pathway choice, promoting non-homologous end joining (NHEJ) pathways, and limiting homologous recombination. 53BP1 plays a crucial role in the DNA damage response, particularly in the repair of double-strand breaks through non-homologous end joining. This function is vital for maintaining genomic stability and preventing tumorigenesis, as failure to properly repair DNA can lead to mutations and cancer development. 53BP1′s ability to promote cell proliferation through interactions with p202, while also having the potential to induce cell death via binding to Bcl2 and NFκB p65, highlights 53BP1′s dual role in cellular processes. This makes 53BP1 a significant target for research in cancer biology and therapeutic development. |
| Product Image Gallery | |
 Western blot analysis of extracts on A549, HEK293 cells, using 53BP1 Rabbit Polyclonal Antibody (EAB13216) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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