| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 36 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Atg3 Rabbit Polyclonal Antibody detects endogenous levels of Atg3 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Atg3(Autophagy related 3) also known as APG3 is a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. Atg3 plays a crucial role in the autophagy process by catalyzing the formation of the Atg8-phosphatidylethanolamine (Atg8-PE) conjugate, which is essential for the degradation of cellular components and organelles. The enzymatic activity of Atg3 is initiated by the removal of the C-terminal arginine residue of Atg8 by Atg4, a cysteine protease, which exposes a glycine residue that is subsequently activated by Atg7. The activated glycine is then transferred to Atg3, where conjugation to phosphatidylethanolamine (PE) occurs. Atg3 is predominantly expressed in tissues such as the kidney, placenta, liver, heart, and skeletal muscle, highlighting its importance in various physiological processes. |
| Product Image Gallery | |
 Western blot analysis of extracts on HEK293 cells, using Atg3 Rabbit Polyclonal Antibody (EAB13678) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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