| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 115 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | ATP2A2/SERCA2 Rabbit Polyclonal Antibody detects endogenous levels of ATP2A2/SERCA2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
SERCA2 also konwn as ATP2A2 is one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of the skeletal muscle. SERCA2 plays a crucial role in calcium homeostasis by mediating calcium ion uptake into the sarcoplasmic reticulum, which is essential for muscle contraction and relaxation. This process maintains low cytoplasmic calcium concentrations, preventing excessive muscle contraction and ensuring proper cardiac function. SERCA2 undergoes tissue-specific processing, resulting in two isoforms: SERCA2a, predominantly found in type I (slow) skeletal, cardiac, and smooth muscle, and SERCA2b, expressed in various cell types. Calcium level regulation by SERCA2 particularly influences cardiac muscle contractility and relaxation, highlighting significance in cardiovascular health. |
| Product Image Gallery | |
 Western blot analysis of extracts on HEK293, Mouse heart cells, using ATP2A2/SERCA2 Rabbit Polyclonal Antibody (EAB13682) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human testis tissue, using ATP2A2/SERCA2 Rabbit Polyclonal Antibody (EAB13682) at 1:200 dilution.
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