| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 24 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | BNIP3L/Nix Rabbit Polyclonal Antibody detects endogenous levels of BNIP3L/Nix protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Nix also known as BNIP3L, Nip3L is a protein that belongs to the pro-apoptotic subfamily within the Bcl-2 family of proteins. Nix is a pro-apoptotic member of the Bcl-2 family, and its function is intricately regulated by post-translational modifications such as phosphorylation and ubiquitination. These modifications are critical for controlling Nix′s stability, subcellular localization, and interactions with other proteins involved in apoptotic signaling pathways. The structural integrity and regulation of Nix through these modifications enable Nix to effectively localize to the mitochondria, where Nix interacts with anti-apoptotic proteins like Bcl-xL to initiate programmed cell death, a vital process for maintaining cellular homeostasis. |
| Product Image Gallery | |
 Western blot analysis of extracts on HCT116 cells, using BNIP3L/Nix Rabbit Polyclonal Antibody (EAB13707) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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