| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, ELISA | 122 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | BUB1 Rabbit Polyclonal Antibody detects endogenous levels of BUB1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
BUB1 is a serine/threonine-protein kinase that play a central role in mitosis. BUB1 plays a critical role in the mitotic checkpoint, a crucial mechanism that ensures proper chromosome segregation during cell division. BUB1 is located at the kinetochores, the protein structures on chromosomes where spindle fibers attach during mitosis. Such localization allows BUB1 to monitor microtubule attachment to kinetochores, ensuring chromosomes are correctly aligned before cell proceeds to anaphase. When BUB1 detects misalignment or improper attachment, a checkpoint response activates to halt the cell cycle, preventing potential genomic instability and aneuploidy. BUB1′s role in maintaining genomic integrity highlights potential applications in cancer research, where mitotic checkpoint dysregulation can lead to tumorigenesis. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, 293T cells, using BUB1 Rabbit Polyclonal Antibody (EAB13711) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 122 kDa; Observed band size: 133 kDa |
