| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P | 29 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | CD27 Rabbit Polyclonal Antibody detects endogenous levels of CD27 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CD27 also known as TNFRSF7, Tp55 is a member of the TNF-receptor superfamily. CD27 plays a crucial role in the immune system as a homodimeric lymphocyte-specific surface antigen found on T and B lymphocytes, facilitating essential co-stimulatory signals necessary for T cell activation when engaged with CD27L. This interaction is vital for T cell proliferation and survival, particularly during immune responses, highlighting CD27′s importance in maintaining effective immune function. Additionally, CD27 expression is upregulated following CD3 complex activation via T cell receptor for antigen, further underscoring CD27′s role in T cell activation and differentiation. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HL60, Jurkat cells, using CD27 Rabbit Polyclonal Antibody (EAB13730) at 1:2000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 29 kDa; Observed band size: 52 kDa |
