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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 35 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | CD47 Rabbit Polyclonal Antibody detects endogenous levels of CD47 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CD47 is a membrane protein that plays a crucial role in regulating cation fluxes across cell membranes, which is vital for maintaining cellular homeostasis and signaling. Specifically, CD47 is involved in the increase of intracellular calcium concentration that occurs upon cell adhesion to the extracellular matrix, a process that is essential for various cellular functions, including migration, proliferation, and survival. Additionally, CD47 serves as a receptor for the C-terminal cell binding domain of thrombospondin, which is important for mediating cell-cell interactions and influencing immune responses. Notably, CD47 is absent from Rh-null erythrocytes, yet CD47 plays a significant role in cell adhesion in non-erythroid cells and may help prevent the premature elimination of erythrocytes. |
| Product Image Gallery | |
 Western blot analysis of extracts on U251, NIH/3T3 cells, using CD47 Rabbit Polyclonal Antibody (EAB13736) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 35 kDa; Observed band size: 47 kDa  Immunohistochemical analysis of paraffin-embedded Human lymph nodes tissue, using CD47 Rabbit Polyclonal Antibody (EAB13736) at 1:100 dilution.
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