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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IP, IHC-P, IF/ICC, ChIP | 83 | Human, Mouse, Rat, Monkey |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IP=1:20-200 IHC-P=1:50-300 IF=1:50-300 ChIP=1:10-100 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | CTCF Rabbit Polyclonal Antibody detects endogenous levels of CTCF protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CTCF is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. CTCF plays a crucial role in gene regulation by recognizing long and diverse DNA sequences, which is essential for maintaining proper gene expression patterns. CTCF′s unique structure, characterized by 11 zinc finger motifs—10 of the C2H2 class and 1 of the C2HC class—enables binding to specific DNA regions, influencing transcription of numerous genes. CTCF acts as a repressor of the c-myc gene and regulates expression of the lysozyme gene, while also interacting with the promoter region of the amyloid beta-protein precursor (APP) gene to enhance transcription. This interaction promotes synapse formation between primary neurons during development, highlighting CTCF′s role in neurodevelopmental processes. |
| Product Image Gallery | |
 Western blot analysis of extracts on 293T, Jurkat cells, using CTCF Rabbit Polyclonal Antibody (EAB13867) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 83 kDa; Observed band size: 140 kDa |
