| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 58 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | CYP7A1 Rabbit Polyclonal Antibody detects endogenous levels of CYP7A1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CYP7A1(Cytochrome P450 family 7 subfamily A member 1) is a member of the cytochrome P450 superfamily of enzymes. CYP7A1 plays a crucial role as the rate-limiting enzyme in bile acid synthesis within the liver, a process essential for digesting and absorbing dietary fats and fat-soluble vitamins. CYP7A1 expression is primarily regulated by bile acid receptor FXR, which responds to bile acid levels in the body, ensuring a balanced metabolic response. Dysregulation of CYP7A1 can lead to various metabolic disorders, including cholesterol gallstone disease and liver dysfunction, highlighting CYP7A1′s importance in maintaining lipid homeostasis. |
| Product Image Gallery | |
 Western blot analysis of extracts on HepG2, Mouse liver cells, using CYP7A1 Rabbit Polyclonal Antibody (EAB13810) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 58 kDa; Observed band size: 50 kDa |
