| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 165 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | DOT1L Rabbit Polyclonal Antibody detects endogenous levels of DOT1L protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
DOT1L also known as DOT1L1, DOT1 or KMT4 is a 1739 amino acid homolog of the yeast DOT1 (disruptor of telomeric silencing-1) protein. Localized to the nucleus and highly expressed in testis, lung and kidney, DOT1L is a histone methyltransferase that transfers methyl groups from S-adenosyl-L-methionine to lysine residues on various substrates, such as nucleosomes or histones. While most histone methyltransferases contain a SET domain through which they confer their enzymatic activity, DOT1L does not contain this characteristic domain and is, therefore, thought to function through a different mechanism. DOT1L can bind with several MLL-fusion partners found in acute leukemia and, through this binding, can promote oncogenesis. Two isoforms of DOT1L are expressed due to alternative splicing events. |
| Product Image Gallery | |
 Western blot analysis of extracts on HEK293 cells, using DOT1L Rabbit Polyclonal Antibody (EAB13833) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 165 kDa; Observed band size: 185 kDa |
