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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 264 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-p300 (Ser1834) Rabbit Polyclonal Antibody detects endogenous levels of p300 protein only when phosphorylated at Ser1834. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
p300 also known as KAT3B, EP300 is a critical transcriptional co-activator involved in regulating gene expression by modifying chromatin structure and facilitating the assembly of transcriptional complexes, which is essential for processes such as cell growth, differentiation, and apoptosis. Located primarily in the nucleus, p300 plays a pivotal role in gene regulation by interacting with various transcription factors and nuclear receptors, thereby influencing numerous cellular pathways and maintaining cellular homeostasis. Structurally, p300 is characterized by multiple domains, including bromodomains and HAT (histone acetyltransferase) domains, which are crucial for acetylating histones and other proteins, thereby modulating gene accessibility and transcriptional activity. |
| Product Image Gallery | |
 Western blot analysis of extracts on U2OS cells, using Phospho-p300 (Ser1834) Rabbit Polyclonal Antibody (EAB13961) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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