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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 42 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | FEN1 Rabbit Polyclonal Antibody detects endogenous levels of FEN1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
FEN1 also known as Rad2 is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. FEN1 plays a crucial role in maintaining genome stability by participating in DNA replication, recombination, and repair processes. FEN1 is essential for the proper processing of Okazaki fragments during DNA replication and is involved in excising damaged DNA segments to correct mismatches during repair. Notably, FEN1 is highly homologous to the yeast RAD2 protein, indicating evolutionary conservation and importance in cellular functions. The C-terminal region of FEN1 interacts with proliferating cell nuclear antigen (PCNA), which enhances exonuclease activity during DNA replication. This interaction ensures efficient and accurate replication of DNA, thereby preventing genomic instability that can lead to various diseases, including cancer. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, COLO205 cells, using FEN1 Rabbit Polyclonal Antibody (EAB13874) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human testis tissue, using FEN1 Rabbit Polyclonal Antibody (EAB13874) at 1:100 dilution.
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