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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 80 | Human, Mouse |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | SKI Rabbit Polyclonal Antibody detects endogenous levels of SKI protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
SKI plays a crucial role in cellular processes as a transcriptional regulator, particularly in the context of TGFβ signaling pathways. SKI protein is primarily located in the nucleus, where SKI protein interacts with Smad proteins to modulate gene expression. This nuclear localization is vital because SKI protein effectively represses Smad-mediated transcriptional activation, thereby inhibiting TGFβ-induced cell growth and extracellular matrix production. SKI protein contains five tandem repeats in its C-terminal domain and two leucine zipper motifs, which are essential for DNA binding capabilities and the formation of homodimers and heterodimers. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat cells, using SKI Rabbit Polyclonal Antibody (EAB13984) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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