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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IF, ELISA | 71 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IF=1:50-300 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | TCF4 Rabbit Polyclonal Antibody detects endogenous levels of TCF4 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
TCF4(Transcription factor 4) also known as ITF2 is a basic helix-loop-helix transcription factor. TCF4 recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. TCF4 plays a crucial role in the regulation of gene expression, particularly in muscle development, by binding to specific DNA motifs such as the immunoglobulin enhancer Mu-E5/KE5-motif and the Ephrussi-box (E-box) element found in SSTR2-INR, thereby acting as a transcriptional activator for muscle-specific genes. TCF4 can form homo- or hetero-oligomers with other myogenic factors like myogenin and MyoD, allowing for a complex regulatory network that can either activate or repress transcription depending on the isoform present. This dynamic interaction is essential for proper muscle differentiation and development, highlighting TCF4′s importance in both embryonic and adult tissues, including the heart, brain, and skeletal muscle. |
| Product Image Gallery | |
 Western blot analysis of extracts on A375, K562 cells, using TCF4 Rabbit Polyclonal Antibody (EAB14024) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 71 kDa; Observed band size: 60 kDa |
