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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB | 42 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | WIF1 Rabbit Polyclonal Antibody detects endogenous levels of WIF1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
WIF1 is a 379-amino acid secreted protein characterized by an N-terminal signal sequence, a 150-amino acid WIF domain, five epidermal growth factor-like repeats, and a 45-amino acid hydrophilic C-terminal domain, which collectively contribute to binding Wnt proteins and modulating their activity. WIF1 plays a crucial role as a Wnt antagonist, which is essential for regulating the Wnt signaling pathway that governs numerous developmental processes such as embryogenesis and central nervous system patterning. By inhibiting Wnt signaling, WIF1 ensures that the spatial and temporal expression of Wnt target genes is properly controlled, preventing aberrant cell proliferation and differentiation that could lead to developmental disorders or cancer. |
| Product Image Gallery | |
 Western blot analysis of extracts on Mouse heart cells, using WIF1 Rabbit Polyclonal Antibody (EAB14133) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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