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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 70 | Human, Mouse |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 | |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-Zap-70 (Tyr292) Rabbit Polyclonal Antibody detects endogenous levels of Zap-70 only when phosphorylated at Tyr292. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Zap-70 also known as SRK, ZAP70 is an enzyme belonging to the protein tyrosine kinase family. Zap-70 plays a crucial role in T cell signaling, particularly in the activation of T lymphocytes following antigen recognition by the T cell receptor (TCR). Upon TCR engagement, Zap-70 is recruited to the receptor complex, where phosphorylation activates downstream signaling pathways that lead to T cell activation, proliferation, and differentiation. This process is vital for the adaptive immune response, enabling T cells to effectively respond to pathogens and maintain immune homeostasis. Zap-70′s importance is underscored by its association with severe immunodeficiency when mutated, highlighting an essential role in T cell function. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat cells treated with UV, using Phospho-Zap-70 (Tyr292) Rabbit Polyclonal Antibody (EAB14113) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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