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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 46 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | GOT1/AATC Rabbit Polyclonal Antibody detects endogenous levels of GOT1/AATC protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
GOT (Glutamic-oxaloacetic transaminase) is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT1 also known as AATC is crucial for amino acid metabolism and plays a significant role in the urea and tricarboxylic acid cycles by catalyzing the conversion of L-aspartate and 2-oxoglutarate into oxaloacetate and L-glutamate. While GOT1and its mitochondrial counterpart AATM share nearly identical three-dimensional structures, GOT1 exhibits distinct expression patterns and folding rates, which can influence interactions with molecular chaperones such as GroEL, underscoring GOT1′s importance in cellular metabolism and potential implications in metabolic disorders. |
| Product Image Gallery | |
 Western blot analysis of extracts on A549, A431 cells, using GOT1/AATC Rabbit Polyclonal Antibody (EAB14129) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry of paraffin-embedded Human brain tissue, using GOT1/AATC Rabbit Polyclonal Antibody (EAB14129) at 1:100 dilution.
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