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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 18 | Human, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Midkine Rabbit Polyclonal Antibody detects endogenous levels of Midkine protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Midkine also known as MK, MDK is a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. Midkine plays a crucial role in regulating growth and differentiation during embryogenesis, making Midkine essential for proper development. Midkine protein is primarily located in the extracellular matrix and is secreted by various cell types, which facilitates cell signaling and interaction with other growth factors. Midkine expression is tightly controlled during embryonic development, influenced by steroid receptors of the retinoic acid superfamily, and Midkine′s mature form consists of 118 amino acids with five intrachain disulfide bonds. Notably, Midkine′s carboxy-terminus harbors the principal heparin-binding site and neurite-promoting sequences, both vital for neurotrophic properties. |
| Product Image Gallery | |
 Western blot analysis of extracts on Raji, PC3, K562 cells, using Midkine Rabbit Polyclonal Antibody (EAB14261) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human brain tissue, using Midkine Rabbit Polyclonal Antibody (EAB14261) at 1:100 dilution.
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