www.ebiocell.com
| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 35 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-MyoD (Ser200) Rabbit Polyclonal Antibody detects endogenous levels of MyoD protein only when phosphorylated at Ser200. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
MyoD is a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. MyoD is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. MyoD plays an essential role in muscle cell differentiation by binding to E-box sequences (CANNTG) in the DNA, thereby regulating the expression of genes critical for muscle development and regeneration. By forming heterodimers with E proteins such as E2A, IF2, and HEB, MyoD activates the transcription of muscle-specific genes, making MyoD a key regulator in myogenesis. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat cells, using Phospho-MyoD (Ser200) Rabbit Polyclonal Antibody (EAB14795) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
|
