www.ebiocell.com
| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 111 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PARG Rabbit Polyclonal Antibody detects endogenous levels of PARG protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PARG(Poly(ADP-ribose) glycohydrolase) is the enzyme responsible for polymer turnover. PARG protein plays a crucial role in cellular response to DNA damage by regulating turnover of poly(ADP-ribose) polymers, which are synthesized by poly(ADP-ribose) polymerase (PARP-1) and other related enzymes. This reversible covalent modification is essential for repair of damaged DNA, as PARG helps modulate activity of chromosomal proteins involved in repair process. Under normal growth conditions, PARG is predominantly localized in cytoplasm and is enzymatically active, undergoing cleavage during apoptosis induced by agents such as etoposide, staurosporine, and Fas. This cleavage generates two C-terminal fragments that retain active site necessary for hydrolyzing poly(ADP-ribose). |
| Product Image Gallery | |
 Western blot analysis of extracts on CACO2 cells, using PARG Rabbit Polyclonal Antibody (EAB14347) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
|
