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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, ELISA | 46 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:1000-5000 IHC-P=1:100-500 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PDK4 Rabbit Polyclonal Antibody detects endogenous levels of PDK4 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PDK4(Pyruvate dehydrogenase kinase 4) is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. PDK4 plays a crucial role in cellular metabolism by regulating the activity of the pyruvate dehydrogenase complex (PDC) through phosphorylation of its E1α subunit. This regulation is vital as PDK4 determines the balance between carbohydrate and fatty acid oxidation, particularly during metabolic stress such as starvation or diabetes. PDK4 is predominantly expressed in heart and skeletal muscle, where PDK4 levels fluctuate in response to nutritional status; for instance, PDK4 levels increase during starvation, leading to reduced PDC activity and a shift towards fatty acid utilization for energy. |
| Product Image Gallery | |
 Western blot analysis of extracts on HepG2, Mouse Heart, Rat Muscle cells, using PDK4 Rabbit Polyclonal Antibody (EAB14350) at 1:2000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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