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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 69 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PCK1/PEPCK-C Rabbit Polyclonal Antibody detects endogenous levels of PCK1/PEPCK-C protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PCK1 also known as PEPCK-C, PEPCK1 is a crucial enzyme in the gluconeogenesis pathway, responsible for the synthesis of glucose from non-carbohydrate sources in liver cells, thereby maintaining blood glucose levels during fasting and metabolic stress. Structurally, PCK1 interacts with several key proteins, including the transcription factor FOXO1 and the coactivator PGC-1α, which regulate its expression in response to hormonal signals like insulin and glucagon. These interactions are essential for the dynamic control of glucose production and metabolic homeostasis. Dysregulation of PCK1 and its interacting partners is closely associated with metabolic disorders such as type 2 diabetes and obesity, highlighting its significance as a therapeutic target. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, MCF7 cells, using PCK1/PEPCK-C Rabbit Polyclonal Antibody (EAB14360) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Mouse liver tissue, using PCK1/PEPCK-C Rabbit Polyclonal Antibody (EAB14360) at 1:100 dilution.
 Immunohistochemical analysis of paraffin-embedded Rat liver tissue, using PCK1/PEPCK-C Rabbit Polyclonal Antibody (EAB14360) at 1:100 dilution.
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