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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 17 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | ID1 Rabbit Polyclonal Antibody detects endogenous levels of ID1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
ID1(Inhibitor of DNA binding 1) is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. ID1 plays a critical role in regulating various cellular processes including differentiation, proliferation, and angiogenesis. Located primarily in the nucleus, Id1 functions by dimerizing with class A and B HLH proteins, thereby inhibiting their ability to bind DNA and negatively regulating their transcriptional activity. This nuclear localization is essential as Id1 controls the expression of genes involved in muscle-specific differentiation by preventing transcription factors like E12 and Myo D from activating myogenic genes. Under conditions that promote muscle cell differentiation, nuclear levels of Id1 decrease, facilitating the formation of active heterodimers between E12 or E47 and Myo D or myogenin, which then drive the differentiation process forward. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat cells, using ID1 Rabbit Polyclonal Antibody (EAB14377) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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