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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 13 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | ID3 Rabbit Polyclonal Antibody detects endogenous levels of ID3 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
ID3 also known as HEIR1 is a member of the Id family of basic helix-loop-helix (bHLH) proteins, ID3 plays a crucial role in cellular differentiation and proliferation by acting as a negative regulator of DNA binding for bHLH proteins. ID3 can dimerize with class A and B HLH proteins, preventing these complexes from binding to DNA and inhibiting gene expression necessary for muscle differentiation. As muscle cells differentiate, Id protein levels decrease, allowing for active heterodimer formation that promotes myogenic differentiation. Growth factors tightly regulate ID3 expression, and studies show reduced Id mRNA levels can delay quiescent cells′ reentry into the cell cycle upon growth factor stimulation, highlighting ID3 ′s significance in developmental processes and potential implications in muscle-related pathologies. |
| Product Image Gallery | |
 Western blot analysis of extracts on HCT116 cells, using ID3 Rabbit Polyclonal Antibody (EAB14379) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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