| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 51 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | IDH2 Rabbit Polyclonal Antibody detects endogenous levels of IDH2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
IDH2 also known as IDHM is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. IDH2 plays a vital role in cellular metabolism by catalyzing the conversion of isocitrate to alpha-ketoglutarate while reducing NADP+ to NADPH, which is essential for maintaining the cellular redox state and supporting the synthesis of glutathione, a key antioxidant. The proper functioning of IDH2 is critical for energy production and intermediary metabolism, as IDH2 helps regulate the balance of metabolic intermediates in the mitochondria. Additionally, IDH2 forms a homodimer in the mitochondrion and can bind one magnesium or manganese ion per subunit, which is important for enzymatic activity. |
| Product Image Gallery | |
 Western blot analysis of extracts on MCF7, Jurkat, HepG2 cells, using IDH2 Rabbit Polyclonal Antibody (EAB14370) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue, using IDH2 Rabbit Polyclonal Antibody (EAB14370) at 1:200 dilution.
|
