| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 70 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | ADAM10 Rabbit Polyclonal Antibody detects endogenous levels of ADAM10 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
ADAM10 also known as KUZ, MADM is a TNF-processing enzyme that cleaves pro-TNF, a membrane-bound precusor protein, at Ala 76-Val 77, which causes membrane shedding of soluble TNF. ADAM10 plays a crucial role in processing tumor necrosis factor alpha (TNFα), a key cytokine involved in inflammation and immune responses. By cleaving membrane-bound precursor pro-TNF at Ala 76-Val 77, ADAM10 facilitates soluble TNF release, which is essential for regulating inflammatory processes and mediating cell signaling pathways. ADAM10 maintains homeostasis in various physiological processes, including immune response and tissue remodeling. ADAM10 expression occurs in a wide range of tissues, including brain, testis, ovary, and heart, highlighting significance in both normal physiology and potential pathophysiological conditions. |
| Product Image Gallery | |
 Western blot analysis of extracts on K562 cells, using ADAM10 Rabbit Polyclonal Antibody (EAB13971) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human lymph tissue, using ADAM10 Rabbit Polyclonal Antibody (EAB13971) at 1:100 dilution.
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