| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 45 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | ACAT1 Rabbit Polyclonal Antibody detects endogenous levels of ACAT1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
ACAT1(Acetyl-CoA acetyltransferase 1) is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. ACAT1 plays a significant role in ketone body metabolism and isoleucine processing. ACAT1 protein is located within mitochondria, where ACAT-1 facilitates acetyl-CoA conversion into acetoacetyl-CoA, a key step in ketone body synthesis and degradation. ACAT1′s mitochondrial localization is important for regulating energy metabolism, particularly during fasting or low carbohydrate intake, when ketone bodies serve as an alternative energy source for brain and heart tissues. Deficiencies in ACAT1 can lead to serious metabolic disorders, including β-ketothiolase deficiency, characterized by ketoacidotic attacks. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, MCF7, A549, HepG2 cells, using ACAT1 Rabbit Polyclonal Antibody (EAB14410) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, using ACAT1 Rabbit Polyclonal Antibody (EAB14410) at 1:200 dilution.
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