| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF | 29 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IHC-P=1:100-500 IF=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | CD16 Mouse Monoclonal Antibody detects endogenous levels of CD16 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CD16 is a crucial immune receptor that exists in two primary isoforms: CD16A (FcγRIIIA), a transmembrane protein expressed on natural killer (NK) cells and macrophages, and CD16B (FcγRIIIB), a glycosylphosphatidylinositol (GPI)-anchored protein found on neutrophils. The distinct structural configurations of these isoforms are essential for their specific roles in the immune response, with CD16A requiring association with the γ subunit of FcεRI or the ζ subunit of the TCR-CD3 complex for proper cell surface expression. This structural versatility allows CD16 to mediate antibody-dependent cellular cytotoxicity (ADCC) and phagocytosis by binding the Fc region of IgG antibodies, thereby targeting and eliminating pathogens or infected cells effectively. CD16′s ability to exist both as a transmembrane and a GPI-anchored receptor highlights versatile functions in immune regulation and response. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat, K562 cells, using CD16 Mouse Monoclonal Antibody (EAB13573) at 1:2000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 29 kDa; Observed band size: 45 kDa  Immunohistochemical analysis of paraffin-embedded Human lung caricnoma tissue, using CD16 Mouse Monoclonal Antibody (EAB13573) at 1:200 dilution.
|
