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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 23 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-RhoGDI (Ser174) Rabbit Polyclonal Antibody detects endogenous levels of RhoGDI protein only when phosphorylated at Ser174. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
RhoGDI also known as RhoGDIα, ARHGDIA is a protein that plays a significant role in regulating Rho GTPases, which are essential for various cellular processes, including cell proliferation and cytoskeletal dynamics. RhoGDI is primarily localized in the cytoplasm, where RhoGDI functions to inhibit the dissociation of guanosine diphosphate (GDP) from Rho proteins, thereby preventing the activation of these GTPases by guanosine triphosphate (GTP). Regulation of RhoGDI is vital, as RhoGDI activity can be modulated by phosphorylation at Ser 101 by p21-activated kinase (αPAK), which can lead to a decrease in inhibitory function and potentially enhance the signaling pathways mediated by Rho proteins. |
| Product Image Gallery | |
 Western blot analysis of extracts on HuvEc cells treated with EGF, using Phospho-RhoGDI (Ser174) Rabbit Polyclonal Antibody (EAB11551) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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