| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, ELISA | 11 | Human, Mouse, Rat, Monkey |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Histone H4 Mouse Monoclonal Antibody detects endogenous levels of Histone H4 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Histone H4 is one of the four core histones (H2A, H2B, H3, and H4) essential for the formation of nucleosomes, which are fundamental units of chromatin structure in eukaryotic cells. Histone H4 plays a crucial role in the regulation of gene expression and chromatin dynamics. Histone H4, along with the other core histones, helps package DNA into nucleosome particles, facilitating the organization of chromosomal fiber and regulating gene expression. The histone H4 protein undergoes various post-translational modifications, including methylation, acetylation, phosphorylation, and ubiquitination, which are crucial for the regulation of chromatin dynamics, DNA repair, and transcription. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, MCF7, Mouse liver cells, using Histone H4 Mouse Monoclonal Antibody (EAB21793) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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