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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 97 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-MCM4 (Ser54) Rabbit Polyclonal Antibody detects endogenous levels of MCM4 protein only when phosphorylated at Ser54. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
MCM4 also known as IMD54 is a member of the mini-chromosome maintenance (MCM) protein family. MCM4 plays a crucial role in the regulation of DNA replication, ensuring that DNA is replicated only once per cell cycle, which is vital for maintaining genomic stability. MCM4 is primarily located in the nucleus, where MCM4 forms part of the MCM2-7 complex that acts as a helicase during the initiation of DNA replication. This localization is essential because MCM4 interacts with other replication factors and DNA, facilitating the unwinding of double-stranded DNA necessary for replication. Furthermore, MCM4 activity is tightly regulated by various mechanisms, including phosphorylation by cyclin-dependent kinases, which ensures that the replication process is precisely timed and coordinated with the cell cycle. |
| Product Image Gallery | |
 Western blot analysis of extracts on K562 cells, using Phospho-MCM4 (Ser54) Rabbit Polyclonal Antibody (EAB11273) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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