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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P | 189 | Human, Mouse |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | IQGAP1 Rabbit Polyclonal Antibody detects endogenous levels of IQGAP1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
IQGAP1 also known as IQ motif containing GTPase activating protein 1 is a member of the IQGAP family. IQGAP1 is an actin-binding protein associated with the cytoskeleton, which is essential for maintaining cell shape and facilitating motility and polarity. Predominantly located at the plasma membrane and cytoskeletal structures, IQGAP1 plays a central role in cellular signaling by interacting with small GTPases, including Cdc42 and Rac1, to regulate the organization of actin filaments. This positioning is crucial as IQGAP1 mediates key processes in cell adhesion, migration, and morphology, making IQGAP1 indispensable in tissues like the lung, placenta, and kidney. Additionally, IQGAP1 modulates cell-cell adhesion by binding to components of the cadherin complex, including E-cadherin, β-catenin, and α-catenin, to stabilize cell junctions. Calmodulin, binding in a calcium-dependent manner, regulates IQGAP1′s activity by preventing association with Cdc42, thus providing dynamic control over cell structure. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, MCF7 cells, using IQGAP1 Rabbit Polyclonal Antibody (EAB14414) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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