| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 47 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Acetyl-E2F1 (Lys117) Rabbit Polyclonal Antibody detects endogenous levels of E2F1 protein only when acetylated at Lys117. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
E2F1 also known as RBP3 is a member of the E2F family of transcription factors. E2F1 plays a critical role in both normal cell cycle control and in the dysregulation of cell proliferation seen in various malignancies. E2F1 forms functional heterodimers with DP-1, and this complex drives the expression of genes that promote cell cycle progression and DNA replication. E2F1 is particularly important in regulating the transition from the G1 phase to the S phase, and E2F1 activity is tightly controlled by interactions with the retinoblastoma protein (Rb). Rb binding inhibits E2F-1 transcriptional activity, preventing the expression of cell cycle genes until appropriate signals trigger Rb inactivation, a process often disrupted in cancer. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Mouse brain cells, using Acetyl-E2F-1 (Lys117) Rabbit Polyclonal Antibody (EAB11509) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 47 kDa; Observed band size: 60 kDa |
