| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 97 | Human, Mouse, Rat, Pig |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:5000-20000 IHC-P=1:500-2000 IF/ICC=1:200-1000 FC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | DAG1 Mouse Monoclonal antibody detects endogenous levels of DAG1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
DAG1 also known as Dystroglycan is a central component of dystrophin-glycoprotein complex that links the extracellular matrix and the cytoskeleton in the skeletal muscle. DAG1 plays a crucial role as a cell surface receptor for several extracellular matrix molecules, including laminins, Agrin, and Perlecan, which are essential for basement membrane formation during early development and laminin organization on cell surfaces. α-DAG1′s location at cell membranes facilitates interaction with dystrophin and utrophin, forming dystrophin-associated protein complex and utrophin-associated protein complex, respectively. These complexes maintain structural integrity of muscle cells and cluster nicotinic acetylcholine receptors at neuromuscular junctions, essential for effective synaptic transmission. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HepG2, Mouse brain cells, using DAG1 Mouse Monoclonal antibody (EAB22523) at 1:10000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 97 kDa; Observed band size: 40 kDa |
