| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 46 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | BCKDK Rabbit Polyclonal Antibody detects endogenous levels of BCKDK protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
BCDKD is an important regulator of the valine, leucine, and isoleucine catabolic pathways. BCDKD plays a vital role in regulating the branched-chain alpha-ketoacid dehydrogenase (BCKD) complex. The BCKD complex catalyzes the irreversible oxidative decarboxylation of BCAAs, making BCKD essential for energy production and metabolic homeostasis. By phosphorylating and inactivating the BCKD complex, BCKDK serves as a critical regulator of BCAA metabolism, influencing various physiological processes, including energy balance and muscle metabolism. BCKDK′s ability to modulate BCKD complex activity underscores BCKDK′s importance in metabolic pathways and highlights BCKDK′s potential as a target for therapeutic interventions in metabolic disorders. |
| Product Image Gallery | |
 Western blot analysis of extracts on K562, Jurkat, HEK293, NIH/3T3 cells, using BCKDK Rabbit Polyclonal Antibody (EAB11059) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, using BCKDK Rabbit Polyclonal Antibody (EAB11059) at 1:200 dilution.
 Immunofluorescence analysis of Hela cell, using BCKDK Rabbit Polyclonal Antibody (EAB11059) at 1:200 dilution.
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