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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 59 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | SPAK Rabbit Polyclonal Antibody detects endogenous levels of SPAK protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
SPAK also known as DCHT, STK39 is a serine/threonine kinase that is thought to function in the cellular stress response pathway. SPAK catalyzes ATP-dependent phosphorylation of target proteins, mediating stress-activated signals essential for regulating various cellular functions, including cell division, homeostasis, and apoptosis. The phosphorylation and dephosphorylation of proteins on serine and threonine residues are crucial for maintaining cellular integrity and responding to environmental changes. The gene encoding SPAK is located on human chromosome 2, which contains over 1,400 genes and represents nearly 8% of the human genome, highlighting SPAK′s significance in human health and disease. |
| Product Image Gallery | |
 Western blot analysis of extracts on COLO205, HepG2 cells, using SPAK Rabbit Polyclonal Antibody (EAB11144) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, using SPAK Rabbit Polyclonal Antibody (EAB11144) at 1:100 dilution.
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