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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 80 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | GRK2 Mouse Monoclonal Antibody detects endogenous levels of GRK2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
GRK2 also known as BARK1, ADRBK1 is a member of the G protein-coupled receptor kinase family of proteins. GRK2 protein plays a crucial role in the regulation of G protein-coupled receptor signaling, which is essential for numerous physiological processes, including vision, taste, and neurotransmission. Located primarily in the cytoplasm and membrane of cells, GRK2 is activated upon receptor stimulation, leading to the phosphorylation of activated receptors. This phosphorylation is vital for the desensitization of receptors, preventing overstimulation and ensuring that cells can respond appropriately to external signals. GRK2 modulation of receptor activity is particularly important in the context of cardiovascular health, as GRK2 helps regulate heart rate and contractility. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat, Molt-4, Raji, THP-1 cells, using GRK2 Mouse Monoclonal Antibody (EAB22605) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution
 Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue, using GRK2 Mouse Monoclonal Antibody (EAB22605) at 1:200 dilution.
 Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue, using GRK2 Mouse Monoclonal Antibody (EAB22605) at 1:200 dilution.
 Immunofluorescence analysis of NIH/3T3 cells, using GRK2 Mouse Monoclonal Antibody (EAB22605)(green) at 1:200 dilution.
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