www.ebiocell.com
| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 25 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 | |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | HAM3C Rabbit Polyclonal Antibody detects endogenous levels of HAM3C protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
FAM3C also known as ILEI is a member of the family with sequence similarity 3 (FAM3) family and encodes a secreted protein with a GG domain. FAM3C plays a crucial role in studying cellular processes, particularly in cancer biology, as FAM3C is implicated in the epithelial to mesenchymal transition (EMT), a key event in tumor progression and metastasis. Elevated levels of FAM3C are observed in oncogenic Ras-transformed mammary epithelial cells, and FAM3C overexpression has been linked to the loss of tight junction protein ZO-1 and the expression of cytoplasmic E-cadherin, which together contribute to increased invasiveness and loss of cellular polarity. The interaction of ILEI with oncogenic Ras suggests a cooperative mechanism that drives TGFβ-independent EMT, making ILEI a significant factor in the invasion, metastasis, and survival of various cancers. |
| Product Image Gallery | |
 Western blot analysis of extracts on SH-SY5Y cells, using HAM3C Rabbit Polyclonal Antibody (EAB11091) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
|
