| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 68 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PIAS3 Rabbit Polyclonal Antibody detects endogenous levels of PIAS3 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PIAS3 also designated ZMIZ5 is a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. PIAS3 plays a crucial role in regulating signal transduction pathways mediated by the IL-6-type family of cytokines, which are essential for gene activation, cell proliferation, and differentiation. Specifically, PIAS3 functions as a negative regulator of the transcription factor Stat3, which is activated in response to cytokine signaling. By binding to Stat3, PIAS3 inhibits DNA-binding activity, thereby preventing transcription of target genes crucial for various cellular processes, including inflammation and immune responses. This regulatory mechanism is vital for maintaining cellular homeostasis and preventing excessive activation of Stat3, which can lead to pathological conditions such as cancer and autoimmune diseases. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HEK293, Rat kidney cells, using PIAS3 Rabbit Polyclonal Antibody (EAB11094) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry analysis of paraffin-embedded Human lung carcinoma tissue, using PIAS3 Rabbit Polyclonal Antibody (EAB11094) at 1:100 dilution.
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