| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 77 | Human, Mouse, Monkey |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Dbf4 Rabbit Polyclonal Antibody detects endogenous levels of Dbf4 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Dbf4 is a protein that plays a crucial role in regulating DNA replication, specifically in forming and activating the Dbf4/Cdc7 kinase complex, which is essential for initiating DNA replication during the S phase of the cell cycle. This complex phosphorylates key proteins necessary for DNA replication initiation, including several minichromosome maintenance proteins and the p180 subunit of DNA polymerase alpha-primase. Dbf4 expression remains tightly regulated throughout the cell cycle, staying absent during G1 phase but accumulating during S and G2 phases, ensuring DNA replication occurs at appropriate times. Timely degradation of Dbf4 by the anaphase-promoting complex during chromosome segregation prevents premature reactivation of prereplicative complexes, maintaining genomic stability. |
| Product Image Gallery | |
 Western blot analysis of extracts on COS7, NIH/3T3 cells, using Dbf4 Rabbit Polyclonal Antibody (EAB11704) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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