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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IF/ICC, ELISA | 57 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:1000-5000 IF/ICC=1:100-500 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | HARS Mouse Monoclonal antibody detects endogenous levels of HARS protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
HARS also known as HisRS, HARS1 is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. HARS plays a crucial role in protein synthesis by charging transfer RNAs (tRNAs) with histidine, which is essential for the incorporation of this amino acid into proteins. Proper functioning of HARS ensures proteins are synthesized correctly, which is particularly important in maintaining cellular functions and overall organismal health. Dysregulation of HARS can lead to the production of autoantibodies in systemic autoimmune diseases, where the immune system mistakenly targets its own proteins. HARS is recognized as an antigen in conditions such as rheumatic arthritis and polymyositis, which can be associated with interstitial lung disease, thrombocytopenia, and Raynaud′s phenomenon, a condition characterized by vasospastic attacks that constrict blood vessels. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Jurkat, HEK293, HepG2 cells, using HARS Mouse Monoclonal antibody (EAB13433) at 1:3000 dilution. Secondary antibody Goat Anti-Mosue IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 57 kDa; Observed band size: 50 kDa |
