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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 141 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 | |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Vigilin Rabbit Polyclonal Antibody detects endogenous levels of Vigilin protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Vigilin also known as HDLBP is found in the nucleus and cytoplasm of all eukaryotic species. where its unique structure, comprising 14 to 15 consecutively arranged KH domains, plays a critical role in mediating RNA-protein interactions. This structural configuration is essential for Vigilin′s function in heterochromatin formation and cytoplasmic mRNA decay, making Vigilin a valuable marker for assessing translational activity. The gene encoding Vigilin, located on chromosome 2q37, is vital for cell viability, and Vigilin expression is upregulated by intracellular cholesterol levels. Notably, Vigilin interacts with the 80S ribosome through its C-terminal domain, linking tRNA export and the channeled tRNA cycle on ribosomes, which underscores its importance in cellular processes related to protein synthesis and lipid metabolism. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat cells, using Vigilin Rabbit Polyclonal Antibody (EAB11177) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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