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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, FC, ELISA | 59 | Human, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 FC=1:50-200 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | TdT Mouse Monoclonal Antibody detects endogenous levels of TdT protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
TdT also known as DNTT is a member of the DNA polymerase type-X family. TdT plays a crucial role in DNA synthesis by catalyzing the addition of deoxyribonucleotides to the 3′-hydroxyl end of DNA primers without the need for a template, which is essential for precise labeling of DNA′s 3′ termini. The human TdT gene is located on chromosome 10q23-q24 and encodes a 510 amino acid protein that is synthesized as a single-chain peptide. This enzyme exhibits a slight preference for incorporating deoxyribonucleotides over ribonucleotides, which is significant for maintaining DNA strand integrity during replication and repair processes. TdT is predominantly found in immature thymocytes, certain bone marrow cells, transformed pre-B and pre-T cell lines, as well as in leukemia cells, highlighting its importance in both normal and pathological cellular contexts. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, A549, PC12 cells, using TdT Mouse Monoclonal Antibody (EAB22695) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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