| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 121 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | NKCC2 Rabbit Polyclonal Antibody detects endogenous levels of NKCC2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
NKCC2 also known as SLC12A1 is a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop and the macula densa. NKCC2 plays a crucial role in the kidney, specifically in the thick ascending limb of the loop of Henle, where NKCC2 is responsible for active reabsorption of sodium and chloride ions. This function is vital for maintaining electrolyte balance and blood pressure regulation in the body. The gene encoding human NKCC2 is located on chromosome 15q15-q21, and mutations in this gene can lead to Bartter′s syndrome, a genetic disorder characterized by hypokalemic alkalosis and metabolic imbalances. NKCC2 is sensitive to diuretics such as furosemide and bumetanide, which are commonly used in clinical settings to manage conditions like hypertension and edema. |
| Product Image Gallery | |
 Western blot analysis of extracts on THP-1, Rat kidney cells, using NCKK2 Rabbit Polyclonal Antibody (EAB11214) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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