| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IF, ELISA | 114 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | ANO1/DOG1 Rabbit Polyclonal Antibody detects endogenous levels of ANO1/DOG1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
ANO1(Anoctamin 1) also known as DOG1, TMEM16A is a 986 amino acid multi-pass membrane protein that plays a crucial role as a calcium-activated chloride channel, essential for normal tracheal development and various physiological processes. ANO1 localization to both cell membrane and cytoplasm facilitates chloride ion transport across membranes, vital for maintaining cellular homeostasis and regulating fluid secretion in epithelial tissues. ANO1 expression is highest in liver, gastrointestinal muscle, and skeletal muscle, and has been implicated in several cancers, including breast, pancreatic, gastric, and uterine cancers, as well as neck, ovarian, and parathyroid tumors, indicating potential role in carcinogenesis. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, BT474, Mouse lung, Mouse kidney cells, using ANO1/DOG1 Rabbit Polyclonal Antibody (EAB11285) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
|
