| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 80 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | APPL1 Rabbit Polyclonal Antibody detects endogenous levels of APPL1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
APPL1 also known as APPL, DIP13α plays a crucial role in cellular signaling and trafficking, particularly in epidermal growth factor (EGF) signaling. APPL1 is essential for signal translocation from plasma membrane to nucleus, where APPL1 interacts with chromatin remodeling complexes such as NuRD/MeCP1, thereby influencing gene expression and cell proliferation. APPL1 regulates the Akt signaling pathway, vital for cell survival and metabolism, by binding to kinase domains of Akt1 and Akt2. APPL1 is predominantly expressed in heart, ovary, skeletal muscle, and pancreas, highlighting APPL1′s importance in various physiological processes. With 54% homology to APPL2, APPL1 serves as a key player in the intricate network of signaling pathways that govern cellular responses to external stimuli. |
| Product Image Gallery | |
 Western blot analysis of extracts on A549, K562 cells, using APPL1 Rabbit Polyclonal Antibody (EAB11316) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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