| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 112 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-TFII-I/GTF2I (Tyr248) Rabbit Polyclonal Antibody detects endogenous levels of TFII-I/GTF2I protein only when phosphorylated at Tyr248. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
TFII-I also known as GTF2I is a phosphoprotein containing six characteristic repeat motifs. TFII-I protein binds to the initiator element (Inr) and E-box element in promoters and functions as a regulator of transcription.TFII-I protein plays a crucial role in regulating gene expression, particularly in TATA-less promoters, which are essential for transcribing many genes that lack a traditional TATA box. This characteristic is particularly important as TATA-less promoters are often found in genes expressed in a tissue-specific manner or in response to specific signaling pathways. TFII-I not only binds to various core promoter elements, such as pyrimidine-rich initiator and E-box elements, but also activates c-fos transcription factor, a key player in MAP kinase signaling pathway. |
| Product Image Gallery | |
 Western blot analysis of extracts on LOVO cells, using Phospho-TFII-I/GTF2I (Tyr248) Rabbit Polyclonal Antibody (EAB11325) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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