| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2b | Monoclonal | WB, IP, IHC-P, IF/ICC, ELISA | 22 | Human, Mouse, Rat, Pig, Chicken, Bovine |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:2000-10000 IP=1:100-500 IHC-P=1:200-1000 IF/ICC=1:200-1000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | GPX4 Mouse Monoclonal Antibody detects endogenous levels of GPX4 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
GPX4(Glutathione peroxidase 4) is a unique member of the glutathione peroxidase family. GPX4 plays a crucial role in cellular protection against oxidative stress by reducing lipid hydroperoxides to their corresponding alcohols and converting free hydrogen peroxide into water, thereby preventing lipid peroxidation of cell membranes. GPX4 is unique among the glutathione peroxidase family as GPX4 contains selenocysteine at its active site, which is essential for catalytic function and is encoded by the stop codon TGA. GPX4 is particularly important in maintaining the integrity of cellular membranes and is involved in various physiological processes, including apoptosis and the regulation of cellular redox status. GPX4′s ability to form high molecular weight oligomers enhances functional capacity, making GPX4 a vital component in the antioxidant defense system of higher vertebrates. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Jurkat, HepG2, HEK293, Mouse brain, Rat brain cells, using GPX4 Mouse Monoclonal Antibody (EAB21375) at 1:5000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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